Western blot analysis of various lysates, using NR2F2 antibody (STJ112289) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NR2F2 antibody (1-100) (STJ112289)

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STJ112289
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR2F2
Gene ID: 7026
Uniprot ID: COT2_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NR2F2 (NP_066285.1).
Immunogen Sequence: MAMVVSTWRDPQDEVPGSQG SQASQAPPVPGPPPGAPHTP QTPGQGGPASTPAQTAAGGQ GGPGGPGSDKQQQQQHIECV VCGDKSSGKHYGQFTCEGCK
Tissue Specificity Ubiquitous. Expressed in the stromal cells of developing fetal ovaries.
Function Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. May be required to establish ovary identity during early gonad development.
Protein Name Coup Transcription Factor 2
Coup-Tf2
Apolipoprotein A-I Regulatory Protein 1
Arp-1
Coup Transcription Factor Ii
Coup-Tf Ii
Nuclear Receptor Subfamily 2 Group F Member 2
Database Links Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Coup Transcription Factor 2 antibody
Anti-Coup-Tf2 antibody
Anti-Apolipoprotein A-I Regulatory Protein 1 antibody
Anti-Arp-1 antibody
Anti-Coup Transcription Factor Ii antibody
Anti-Coup-Tf Ii antibody
Anti-Nuclear Receptor Subfamily 2 Group F Member 2 antibody
Anti-NR2F2 antibody
Anti-ARP1 antibody
Anti-TFCOUP2 antibody

Information sourced from Uniprot.org

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