Anti-NR1H3 antibody (151-200 aa) (STJ96508)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human NR1H3 at the amino acid range 151-200
General Information
| Short Description | Rabbit polyclonal anti-Oxysterols receptor LXR-alpha (151-200 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC-P 1:100-300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NR1H3 |
| Gene ID | 10062 |
| Uniprot ID | NR1H3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human NR1H3 at the amino acid range 151-200 |
| Immunogen Region | 151-200 aa |
| Specificity | LXR Alpha Polyclonal Antibody detects endogenous levels of LXR Alpha protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by UBR5, leading to its degradation: UBR5 specifically recognizes and binds ligand-bound NR1H3 when it is not associated with coactivators (NCOAs). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation. |
| Function | Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. |
| Protein Name | Oxysterols Receptor Lxr-AlphaLiver X Receptor AlphaNuclear Receptor Subfamily 1 Group H Member 3 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-383280Reactome: R-HSA-4090294 Q13133-1Reactome: R-HSA-8866427Reactome: R-HSA-9029558Reactome: R-HSA-9029569Reactome: R-HSA-9031525Reactome: R-HSA-9031528Reactome: R-HSA-9623433Reactome: R-HSA-9632974 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Oxysterols Receptor Lxr-Alpha antibodyAnti-Liver X Receptor Alpha antibodyAnti-Nuclear Receptor Subfamily 1 Group H Member 3 antibodyAnti-NR1H3 antibodyAnti-LXRA antibody |
Information sourced from Uniprot.org