• STJ72517 (0. 3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72517 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-NQO1-Isoform a antibody (Internal) (STJ72517)

SKU:
STJ72517-100

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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NQO1-Isoform a (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm of HepG2 cells. 5µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NQO1
Gene ID: 1728
Uniprot ID: NQO1_HUMAN
Immunogen Region: Internal
Accession Number: NP_000894.1
Specificity: This antibody is expected to recognize reported isoform a (NP_000894.1) only.
Immunogen Sequence: DKGPFRSKKAVLS
Protein Name Nad(Ph Dehydrogenase Quinone 1
Azoreductase
Dt-Diaphorase
Dtd
Menadione Reductase
Nad(Ph -Quinone Oxidoreductase 1
Phylloquinone Reductase
Quinone Reductase 1
Qr1
Database Links Reactome: R-HSA-350562
Reactome: R-HSA-9818027
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Nad(Ph Dehydrogenase Quinone 1 antibody
Anti-Azoreductase antibody
Anti-Dt-Diaphorase antibody
Anti-Dtd antibody
Anti-Menadione Reductase antibody
Anti-Nad(Ph -Quinone Oxidoreductase 1 antibody
Anti-Phylloquinone Reductase antibody
Anti-Quinone Reductase 1 antibody
Anti-Qr1 antibody
Anti-NQO1 antibody
Anti-DIA4 antibody
Anti-NMOR1 antibody

Information sourced from Uniprot.org

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