• Western blot analysis of extracts of SH-SY5Y cells, using NPY antibody (STJ24802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NPY rabbit polyclonal antibody (STJ24802) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of SH-SY5Y cells using NPY rabbit polyclonal antibody (STJ24802) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NPY antibody (29-97) (STJ24802)

SKU:
STJ24802

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPY (29-97) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPY
Gene ID: 4852
Uniprot ID: NPY_HUMAN
Immunogen Region: 29-97
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-97 of NPY (NP_000896.1).
Immunogen Sequence: YPSKPDNPGEDAPAEDMARY YSALRHYINLITRQRYGKRS SPETLISDLLMRESTENVPR TRLEDPAMW
Tissue Specificity One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.
Post Translational Modifications The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
Function NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Protein Name Pro-Neuropeptide Y Cleaved Into - Neuropeptide Y
Neuropeptide Tyrosine
Npy - C-Flanking Peptide Of Npy
Cpon
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Neuronal Dense Core Vesicle
Alternative Antibody Names Anti-Pro-Neuropeptide Y Cleaved Into - Neuropeptide Y antibody
Anti-Neuropeptide Tyrosine antibody
Anti-Npy - C-Flanking Peptide Of Npy antibody
Anti-Cpon antibody
Anti-NPY antibody

Information sourced from Uniprot.org

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