Anti-NPM1 antibody [7H10B9] (STJ97858)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant fragment of human B23 expressed in E. Coli.
General Information
| Short Description | Mouse monoclonal anti-Nucleophosmin for use in WB, IHC, IF and ELISA in Human and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Mouse |
| Reactivity | Human/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 7H10B9 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPM1 |
| Gene ID | 4869 |
| Uniprot ID | NPM_HUMAN |
| Immunogen | Purified recombinant fragment of human B23 expressed in E. Coli. |
| Specificity | B23 Monoclonal Antibody detects endogenous levels of B23 protein. |
Additional Info
| Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes. May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1. |
| Protein Name | NucleophosminNpmNucleolar Phosphoprotein B23Nucleolar Protein No38Numatrin |
| Database Links | Reactome: R-HSA-180746Reactome: R-HSA-3899300Reactome: R-HSA-606279Reactome: R-HSA-6804115Reactome: R-HSA-8869496Reactome: R-HSA-9692914Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371Reactome: R-HSA-9833482 |
| Cellular Localisation | NucleusNucleolusNucleoplasmCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeGenerally NucleolarBut Is Translocated To The Nucleoplasm In Case Of Serum Starvation Or Treatment With Anticancer DrugsHas Been Found In The Cytoplasm In Patients With Primary Acute Myelogenous Leukemia (Aml)But Not With Secondary AmlCo-Localizes With The Methylated Form Of Rps10 In The Granular Component (Gc) Region Of The NucleolusColocalized With Nucleolin And Apex1 In NucleoliIsoform 1 Of Nek2 Is Required For Its Localization To The Centrosome During MitosisCan Shuttle Between Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Nucleophosmin antibodyAnti-Npm antibodyAnti-Nucleolar Phosphoprotein B23 antibodyAnti-Nucleolar Protein No38 antibodyAnti-Numatrin antibodyAnti-NPM1 antibodyAnti-NPM antibody |
Information sourced from Uniprot.org