| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NOS3 |
| Gene ID: | 4846 |
| Uniprot ID: | NOS3_HUMAN |
| Immunogen Region: | 639-738 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 639-738 of human eNOS (NP_000594.2). |
| Immunogen Sequence: | AGALGTLRFCVFGLGSRAYP HFCAFARAVDTRLEELGGER LLQLGQGDELCGQEEAFRGW AQAAFQAACETFCVGEDAKA AARDIFSPKRSWKRQRYRLS |
| Tissue Specificity | Platelets, placenta, liver and kidney. |
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. |
| Protein Name | Nitric Oxide Synthase 3 Constitutive Nos Cnos Ec-Nos Nos Type Iii Nosiii Nitric Oxide Synthase - Endothelial Endothelial Nos Enos |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-1474151 Reactome: R-HSA-203615 Reactome: R-HSA-203641 Reactome: R-HSA-203754 Reactome: R-HSA-392154 Reactome: R-HSA-5218920 Reactome: R-HSA-9009391 |
| Cellular Localisation | Cell Membrane Membrane Caveola Cytoplasm Cytoskeleton Golgi Apparatus Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase 3 antibody Anti-Constitutive Nos antibody Anti-Cnos antibody Anti-Ec-Nos antibody Anti-Nos Type Iii antibody Anti-Nosiii antibody Anti-Nitric Oxide Synthase - Endothelial antibody Anti-Endothelial Nos antibody Anti-Enos antibody Anti-NOS3 antibody |
Information sourced from Uniprot.org