| Host: | Mouse |
| Applications: | WB/ICC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-iNOS (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M398 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:250 ICC 1:50 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NOS2 |
| Gene ID: | 4843 |
| Uniprot ID: | NOS2_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFN Gamma and LPS, or J774A.1 cells treated with LPS only. |
| Immunogen: | Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS. |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - Inducible Hepatocyte Nos Hep-Nos Inducible No Synthase Inducible Nos Inos Nos Type Ii Peptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-6785807 Reactome: R-HSA-9033241 Reactome: R-HSA-9636249 |
| Cellular Localisation | Cytoplasm Cytosol Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4 Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibody Anti-Hepatocyte Nos antibody Anti-Hep-Nos antibody Anti-Inducible No Synthase antibody Anti-Inducible Nos antibody Anti-Inos antibody Anti-Nos Type Ii antibody Anti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibody Anti-NOS2 antibody Anti-NOS2A antibody |
Information sourced from Uniprot.org