| Host: | Rabbit |
| Applications: | ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-NOS2 (1064-1114) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55-0.75 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | NOS2 |
| Gene ID: | 4843 |
| Uniprot ID: | NOS2_HUMAN |
| Immunogen Region: | 1064-1114 |
| Specificity: | This antibody will detect both iNOS and iNOS2 protein. |
| Immunogen: | Synthetic peptide taken within amino acid region 1064-1114 on human inducible nitric oxide synthase protein. |
| Tissue Specificity | Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes. |
| Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
| Protein Name | Nitric Oxide Synthase - Inducible Hepatocyte Nos Hep-Nos Inducible No Synthase Inducible Nos Inos Nos Type Ii Peptidyl-Cysteine S-Nitrosylase Nos2 |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-6785807 Reactome: R-HSA-9033241 Reactome: R-HSA-9636249 |
| Cellular Localisation | Cytoplasm Cytosol Localizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4 Exhibits A More Diffuse Cytosolic Localization |
| Alternative Antibody Names | Anti-Nitric Oxide Synthase - Inducible antibody Anti-Hepatocyte Nos antibody Anti-Hep-Nos antibody Anti-Inducible No Synthase antibody Anti-Inducible Nos antibody Anti-Inos antibody Anti-Nos Type Ii antibody Anti-Peptidyl-Cysteine S-Nitrosylase Nos2 antibody Anti-NOS2 antibody Anti-NOS2A antibody |
Information sourced from Uniprot.org