• Immunohistochemistry analysis of paraffin-embedded rat brain using NOMO1 Rabbit polyclonal antibody (STJ112533) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using NOMO1 Rabbit polyclonal antibody (STJ112533) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NOMO1 antibody (STJ112533) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NOMO1 antibody (700-1000) (STJ112533)

SKU:
STJ112533

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NOMO1 (700-1000) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOMO1
Gene ID: 23420
Uniprot ID: NOMO1_HUMAN
Immunogen Region: 700-1000
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 700-1000 of human NOMO1 (NP_055102.3).
Immunogen Sequence: KSVQELRREQQLAEIEARRQ EREKNGNEEGEERMTKPPVQ EMVDELQGPFSYDFSYWARS GEKITVTPSSKELLFYPPSM EAVVSGESCPGKLIEIHGKA GLFLEGQIHPELEGVEIVIS EKGASSPLITVFTDDKGAYS VGPLHSDLEYTVTSQKEGYV LTAVEGTIGDFKAYALAGVS FEIKAEDDQPLPGVLLSLSG GLFRSNLLTQDNGILTFSNL SPGQYYFKPMMKEFRFEPS
Tissue Specificity Expressed in colon tumor tissue and in adjacent normal colonic mucosa.
Function Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions.
Protein Name Bos Complex Subunit Nomo1
Nodal Modulator 1
Pm5 Protein
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Bos Complex Subunit Nomo1 antibody
Anti-Nodal Modulator 1 antibody
Anti-Pm5 Protein antibody
Anti-NOMO1 antibody
Anti-PM5 antibody

Information sourced from Uniprot.org

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