• STJ70967 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70967 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-NMDA receptor 1/GRIN1 antibody (Internal) (STJ70967)

SKU:
STJ70967-100

£312.50
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Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NMDA receptor 1/GRIN1 (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the membrane and cytoplasm of MCF7 and NIH3T3 cells. Recommended concentration: 10µg/ml.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GRIN1
Gene ID: 2902
Uniprot ID: NMDZ1_HUMAN
Immunogen Region: Internal
Accession Number: NP_000823.4; NP_067544.1; NP_015566.1
Specificity: This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
Immunogen Sequence: TQERVNNSNKKE
Function Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium. Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators.
Protein Name Glutamate Receptor Ionotropic - Nmda 1
Glun1
Glutamate Nmda Receptor Subunit Zeta-1
N-Methyl-D-Aspartate Receptor Subunit Nr1
Nmd-R1
Hnr1
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-6794361
Reactome: R-HSA-8849932
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Postsynaptic Density Membrane
Synaptic Cell Membrane
Synaptic Cell Membrane Targeting Is Dependent Of Grin2b/Glun2b Subunit
Association With Grin3a Occurs In The Endoplasmic Reticulum
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 1 antibody
Anti-Glun1 antibody
Anti-Glutamate Nmda Receptor Subunit Zeta-1 antibody
Anti-N-Methyl-D-Aspartate Receptor Subunit Nr1 antibody
Anti-Nmd-R1 antibody
Anti-Hnr1 antibody
Anti-GRIN1 antibody
Anti-NMDAR1 antibody

Information sourced from Uniprot.org

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