• Western blot analysis of lysates from Mouse lung, using NKX2-1 Rabbit monoclonal antibody (STJ11104773) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NKX2-1 antibody (20-110) [S4773RM] (STJ11104773)

SKU:
STJ11104773

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Homeobox Protein Nkx-2.1 (20-110) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4773RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NKX2-1
Gene ID: 7080
Uniprot ID: NKX21_HUMAN
Immunogen Region: 20-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human NKX2-1 (NP_003308.1).
Immunogen Sequence: LEESYKKVGMEGGGLGAPLA AYRQGQAAPPTAAMQQHAVG HHGAVTAAYHMTAAGVPQLS HSAVGGYCNGNLGNMSELPP YQDTMRNSASG
Tissue Specificity Thyroid and lung.
Post Translational Modifications Phosphorylated on serine residues by STK3/MST2.
Function Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1.
Protein Name Homeobox Protein Nkx-2.1
Homeobox Protein Nk-2 Homolog A
Thyroid Nuclear Factor 1
Thyroid Transcription Factor 1
Ttf-1
Thyroid-Specific Enhancer-Binding Protein
T/Ebp
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Nkx-2.1 antibody
Anti-Homeobox Protein Nk-2 Homolog A antibody
Anti-Thyroid Nuclear Factor 1 antibody
Anti-Thyroid Transcription Factor 1 antibody
Anti-Ttf-1 antibody
Anti-Thyroid-Specific Enhancer-Binding Protein antibody
Anti-T/Ebp antibody
Anti-NKX2-1 antibody
Anti-NKX2A antibody
Anti-TITF1 antibody
Anti-TTF1 antibody

Information sourced from Uniprot.org

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