| Short Description : | Rabbit polyclonal NIPSNAP1 (70-145) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NIPSNAP1 |
| Gene ID: | 8508 |
| Uniprot ID: | NIPS1_HUMAN |
| Immunogen Region: | 70-145 |
| Immunogen Sequence: | NLYKIQFHNVKPEYLDAYNS LTEAVLPKLHLDEDYPCSLV GNWNTWYGEQDQAVHLWRFS GGYPALMDCMNKLKNN |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-145 of human NIPSNAP1 (NP_003625.2). |
| Tissue Specificity | Ubiquitous. Highest expression in liver. |
| Post Translational Modifications | In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria. Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2). |
| Protein Name | Protein Nipsnap Homolog 1Nipsnap1 |
| Database Links | |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Protein Nipsnap Homolog 1 antibodyAnti-Nipsnap1 antibodyAnti-NIPSNAP1 antibody |
Information sourced from Uniprot.org
