• Western blot analysis of extracts of various cell lines, using NID1 antibody (STJ110647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using NID1 antibody (STJ110647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-NID1 antibody (110-400) (STJ110647)

SKU:
STJ110647

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NID1
Gene ID: 4811
Uniprot ID: NID1_HUMAN
Immunogen Region: 110-400
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 110-400 of human NID1 (NP_002499.2).
Immunogen Sequence: DLDTTDGLGKVYYREDLSPS ITQRAAECVHRGFPEISFQP SSAVVVTWESVAPYQGPSRD PDQKGKRNTFQAVLASSDSS SYAIFLYPEDGLQFHTTFSK KENNQVPAVVAFSQGSVGFL WKSNGAYNIFANDRESVENL AKSSNSGQQGVWVFEIGSPA TTNGVVPADVILGTEDGAEY DDEDEDYDLATTRLGLEDVG TTPFSYKALRRGGADTYSVP SVLSPRRAATERPLGPPTE
Post Translational Modifications N- and O-glycosylated.
Function Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Protein Name Nidogen-1
Nid-1
Entactin
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-3000157
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Alternative Antibody Names Anti-Nidogen-1 antibody
Anti-Nid-1 antibody
Anti-Entactin antibody
Anti-NID1 antibody
Anti-NID antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance