Western blot with Anti-Nicotinic Acetylcholine Receptor alpha 7 subunit 2 (STJ501864) on 50 pg total rat brain homogenate prepared with buffer with protease inhibitor cocktail. SDS-PAGE 10% with 5% Stacker. Transferred on nitrocellulose using semi-dry method. Blocking for 1 hour at room temperature.

Anti-Nicotinic Acetylcholine Receptor alpha 7 subunit 2 antibody (7-2) (STJ501864)

SKU:
STJ501864-100
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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit polyclonal antibody anti-Nicotinic Acetylcholine Receptor alpha 7 subunit 2 (7-2) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide).
Purification: Affinity Purified
Concentration: 0.75-0.95 µg/µl
Dilution Range: WB: 1:250
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:200
IHC: 1:50-1:150
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Chrna7
Gene ID: 25302
Uniprot ID: ACHA7_RAT
Immunogen Region: 7-2
Immunogen: Synthetic peptide corresponding to exon 5 of alpha 7-2 nAChRs protein.
Post Translational Modifications Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane.
Function After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Protein Name Neuronal Acetylcholine Receptor Subunit Alpha-7
Database Links Reactome: R-RNO-629594
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Ric3 Promotes Its Trafficking To The Cell Membrane
Tmem35a/Nacho Promotes Its Trafficking To The Cell Membrane
Alternative Antibody Names Anti-Neuronal Acetylcholine Receptor Subunit Alpha-7 antibody
Anti-Chrna7 antibody
Anti-Acra7 antibody

Information sourced from Uniprot.org

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