| Short Description : | Rabbit monoclonal anti-Nicastrin for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ICC/IF |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | R02-8L5 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Affinity Chromatography |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | NCSTN |
| Gene ID: | 23385 |
| Uniprot ID: | NICA_HUMAN |
| Immunogen: | A synthesized peptide derived from human Nicastrin |
| Tissue Specificity | Detected in brain (at protein level). Widely expressed. |
| Post Translational Modifications | N-glycosylated. |
| Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. |
| Protein Name | Nicastrin |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-1474228Reactome: R-HSA-193692Reactome: R-HSA-205043Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-3928665Reactome: R-HSA-6798695Reactome: R-HSA-9013507Reactome: R-HSA-9013700Reactome: R-HSA-9017802Reactome: R-HSA-977225Reactome: R-HSA-9839383 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCytoplasmic Vesicle MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Nicastrin antibodyAnti-NCSTN antibodyAnti-KIAA0253 antibodyAnti-UNQ1874 antibodyAnti-PRO4317 antibody |
Information sourced from Uniprot.org
