• Western blot analysis of various cells using NF-YA Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from HepG2, 293, and COLO205 cells, using NFYA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, NF-YA Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-NFYA antibody (261-310) (STJ94455)

SKU:
STJ94455

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nuclear transcription factor Y subunit alpha (261-310) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFYA
Gene ID: 4800
Uniprot ID: NFYA_HUMAN
Immunogen Region: 261-310
Specificity: NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NFYA. AA range:261-310
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.
Protein Name Nuclear Transcription Factor Y Subunit Alpha
Caat Box Dna-Binding Protein Subunit A
Nuclear Transcription Factor Y Subunit A
Nf-Ya
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Transcription Factor Y Subunit Alpha antibody
Anti-Caat Box Dna-Binding Protein Subunit A antibody
Anti-Nuclear Transcription Factor Y Subunit A antibody
Anti-Nf-Ya antibody
Anti-NFYA antibody

Information sourced from Uniprot.org

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