| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NFKB2 |
| Gene ID: | 4791 |
| Uniprot ID: | NFKB2_HUMAN |
| Immunogen Region: | 690-899 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 690-899 of human NF-Kappa B2 (NP_002493.3). |
| Immunogen Sequence: | AGADIHAENEEPLCPLPSPP TSDSDSDSEGPEKDTRSSFR GHTPLDLTCSTKVKTLLLNA AQNTMEPPLTPPSPAGPGLS LGDTALQNLEQLLDGPEAQG SWAELAERLGLRSLVDTYRQ TTSPSGSLLRSYELAGGDLA GLLEALSDMGLEEGVRLLRG PETRDKLPSTEVKEDSAYGS QSVEQEAEKLGPPPEPPGGL CHGHPQPQVH |
| Post Translational Modifications | While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain. Ubiquitinated by TRIM55.leading to processing by VCP and subsequent ubiquitin-dependent protein degradation by the proteasome. |
| Function | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form.the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. |
| Protein Name | Nuclear Factor Nf-Kappa-B P100 Subunit Dna-Binding Factor Kbf2 H2tf1 Lymphocyte Translocation Chromosome 10 Protein Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 Oncogene Lyt-10 Lyt10 Cleaved Into - Nuclear Factor Nf-Kappa-B P52 Subunit |
| Database Links | Reactome: R-HSA-1810476 Reactome: R-HSA-3134963 Reactome: R-HSA-3214841 Reactome: R-HSA-445989 Reactome: R-HSA-448706 Reactome: R-HSA-4755510 Reactome: R-HSA-5603029 Reactome: R-HSA-5607761 Reactome: R-HSA-5676590 Reactome: R-HSA-844456 Reactome: R-HSA-933542 Reactome: R-HSA-9660826 |
| Cellular Localisation | Nucleus Cytoplasm Nuclear But Also Found In The Cytoplasm In An Inactive Form Complexed To An Inhibitor (I-Kappa-B) |
| Alternative Antibody Names | Anti-Nuclear Factor Nf-Kappa-B P100 Subunit antibody Anti-Dna-Binding Factor Kbf2 antibody Anti-H2tf1 antibody Anti-Lymphocyte Translocation Chromosome 10 Protein antibody Anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 antibody Anti-Oncogene Lyt-10 antibody Anti-Lyt10 Cleaved Into - Nuclear Factor Nf-Kappa-B P52 Subunit antibody Anti-NFKB2 antibody Anti-LYT10 antibody |
Information sourced from Uniprot.org