Anti-NFAT5 antibody [1D6] (STJ196652)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenSynthesized peptide derived from the human NFAT5
STJ196652
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General Information

Short DescriptionMouse monoclonal anti-Nuclear factor of activated T-cells 5 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone ID1D6
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution RangeIHC-P 1:50-200
WB 1:1000-2000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNFAT5
Gene ID10725
Uniprot IDNFAT5_HUMAN
ImmunogenSynthesized peptide derived from the human NFAT5
SpecificityThis antibody detects endogenous levels of NFAT5 at Human, Mouse, Rat

Additional Info

Post Translational Modifications Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage.
Function Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.
Protein Name Nuclear Factor Of Activated T-Cells 5
Nf-At5
T-Cell Transcription Factor Nfat5
Tonicity-Responsive Enhancer-Binding Protein
Tone-Binding Protein
Tonebp
Database Links
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Nuclear Distribution Increases Under Hypertonic Conditions
Recruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells 5 antibody
Anti-Nf-At5 antibody
Anti-T-Cell Transcription Factor Nfat5 antibody
Anti-Tonicity-Responsive Enhancer-Binding Protein antibody
Anti-Tone-Binding Protein antibody
Anti-Tonebp antibody
Anti-NFAT5 antibody
Anti-KIAA0827 antibody
Anti-TONEBP antibody

Information sourced from Uniprot.org

Citations

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