| Host: | Mouse |
| Applications: | IF/ICC/IHC/WB |
| Reactivity: | Bovine/Human/Mouse/Pig/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Neurofilament light polypeptide (N-Term) for use in IF, ICC, IHC and WB in Bovine, Human, Mouse, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 6H112 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Purification: | IgG |
| Dilution Range: | WB 3.5138888888888888IHC 2.8194444444444446ICC 0.73611111111111116 |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antib |
| Gene Symbol: | NEFL |
| Gene ID: | 4747 |
| Uniprot ID: | NFL_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | Species cross-reactivity includes human, rat, mouse, cow, and pig. |
| Immunogen: | The antibody has been made against the C-terminal peptide of human NF-L protein (amino acids 514-542; peptide GEEEDTKESEEEEKKEESAGEEQVAKKKD) with an N-terminal C added for coupling to KLH. |
| Immunogen Sequence: | Human |
| Post Translational Modifications | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Light PolypeptideNf-L68 Kda Neurofilament ProteinNeurofilament Triplet L Protein |
| Database Links | Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell ProjectionAxonCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Neurofilament Light Polypeptide antibodyAnti-Nf-L antibodyAnti-68 Kda Neurofilament Protein antibodyAnti-Neurofilament Triplet L Protein antibodyAnti-NEFL antibodyAnti-NF68 antibodyAnti-NFL antibody |
Information sourced from Uniprot.org