Anti-Neurofilament heavy polypeptide, phosphorylated antibody (STJA0040651)
SPECIFICATIONS
ClonalityPolyclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenThis antibody has been made against biochemically isolated NF-H purified from bovine spinal cord.
General Information
| Short Description | Chicken polyclonal anti-Neurofilament heavy polypeptide, phosphorylated for use in ELISA, IF, ICC, IHC and WB in Bovine, Dog, Horse, Human, Mouse, Other Mammals, Pig and Rat samples. Datasheet included with dilution recommendations, and related reage |
| Applications | ELISA/IF/ICC/IHC/WB |
| Host | Chicken |
| Reactivity | Bovine/Dog/Horse/Human/Mouse/Other Mammals/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Purification | IgY preparation |
| Dilution Range | WB 1:20000-1:50000IHC 1:20000ICC 1:20000 |
| Formulation | Lyophilized IgY preparation, with sodium azide. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 50 ยตL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8ยฐC After reconstitution of lyophilized antibody, aliquot and store at-20ยฐC for |
Target Information
| Gene Symbol | NEFH |
| Gene ID | 4744 |
| Uniprot ID | NFH_HUMAN |
| Immunogen | This antibody has been made against biochemically isolated NF-H purified from bovine spinal cord. |
| Immunogen Sequence | Bovine |
| Specificity | Species cross-reactivity includes human, rat, mouse, cow, pig, horse and dog. This antibody reacts with phosphorylated NF-H and is seen as a band at approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an EL |
Additional Info
| Post Translational Modifications | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Heavy PolypeptideNf-H200 Kda Neurofilament ProteinNeurofilament Triplet H Protein |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative Antibody Names | Anti-Neurofilament Heavy Polypeptide antibodyAnti-Nf-H antibodyAnti-200 Kda Neurofilament Protein antibodyAnti-Neurofilament Triplet H Protein antibodyAnti-NEFH antibodyAnti-KIAA0845 antibodyAnti-NFH antibody |
Information sourced from Uniprot.org