STJ70811 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane and secreted staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Netrin 1 antibody (C-Term) (STJ70811)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70811-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Netrin 1 (C-Term) is suitable for use in ELISA and Immunofluorescence research applications.
ApplicationsPep-ELISA/IF
HostGoat
ReactivityHuman/Mouse/Rat/Dog/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the membrane and cytoplasm of U2OS cells. Recommended concentration: 10ยตg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolNTN1
Gene ID9423
Uniprot IDNET1_HUMAN
Accession NumberNP_004813.2
Immunogen RegionC-Term
Immunogen SequenceKFQQREKKGKCKKA

Additional Info

Function Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
Protein Name Netrin-1
Epididymis Tissue Protein Li 131p
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-376172
Reactome: R-HSA-418885
Reactome: R-HSA-418886
Reactome: R-HSA-418890
Reactome: R-HSA-428542
Cellular Localisation Secreted
Cytoplasm
Mainly Secreted
Alternative Antibody Names Anti-Netrin-1 antibody
Anti-Epididymis Tissue Protein Li 131p antibody
Anti-NTN1 antibody
Anti-NTN1L antibody

Information sourced from Uniprot.org

Citations

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