| Short Description : | Rabbit monoclonal anti-Nestin for use in IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | IHC-P/ICC/IF |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | R07-7K3 |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Affinity Chromatography |
| Dilution Range: | IHC 1:50-1:100IF 1:50-1:200 |
| Formulation: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | NES |
| Gene ID: | 10763 |
| Uniprot ID: | NEST_HUMAN |
| Immunogen: | A synthesized peptide derived from human Nestin |
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org
