• Western blot analysis of extracts of Neuro-2a cells, using Nestin antibody (STJ113858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Nestin antibody (STJ113858) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Rat brain using Nestin antibody (STJ113858) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Neuro-2a cells using Nestin rabbit polyclonal antibody (STJ113858) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NES antibody (1392-1621) (STJ113858)

SKU:
STJ113858

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nestin (1392-1621) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NES
Gene ID: 10763
Uniprot ID: NEST_HUMAN
Immunogen Region: 1392-1621
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1392-1621 of human NES (NP_006608.1).
Immunogen Sequence: QVPQLLLDPAAWDRDGESDG FADEEESGEEGEEDQEEGRE PGAGRWGPGSSVGSLQALSS SQRGEFLESDSVSVSVPWDD SLRGAVAGAPKTALETESQD SAEPSGSEEESDPVSLERED KVPGPLEIPSGMEDAGPGAD IIGVNGQGPNLEGKSQHVNG GVMNGLEQSEEVGQGMPLVS EGDRGSPFQEEEGSALKTSW AGAPVHLGQGQFLKFTQREG DRESWSSGED
Tissue Specificity CNS stem cells.
Post Translational Modifications Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Protein Name Nestin
Alternative Antibody Names Anti-Nestin antibody
Anti-NES antibody
Anti-Nbla00170 antibody

Information sourced from Uniprot.org

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