| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NES |
| Gene ID: | 10763 |
| Uniprot ID: | NEST_HUMAN |
| Immunogen Region: | 1392-1621 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1392-1621 of human NES (NP_006608.1). |
| Immunogen Sequence: | QVPQLLLDPAAWDRDGESDG FADEEESGEEGEEDQEEGRE PGAGRWGPGSSVGSLQALSS SQRGEFLESDSVSVSVPWDD SLRGAVAGAPKTALETESQD SAEPSGSEEESDPVSLERED KVPGPLEIPSGMEDAGPGAD IIGVNGQGPNLEGKSQHVNG GVMNGLEQSEEVGQGMPLVS EGDRGSPFQEEEGSALKTSW AGAPVHLGQGQFLKFTQREG DRESWSSGED |
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org
