| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Serine/threonine-protein kinase Nek6 (90-170 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NEK6 |
| Gene ID: | 10783 |
| Uniprot ID: | NEK6_HUMAN |
| Immunogen Region: | 90-170 aa |
| Specificity: | NEK6 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 90-170 |
| Post Translational Modifications | Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210. |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. |
| Protein Name | Serine/Threonine-Protein Kinase Nek6 Never In Mitosis A-Related Kinase 6 Nima-Related Protein Kinase 6 Protein Kinase Sid6-1512 |
| Database Links | Reactome: R-HSA-2980767 Reactome: R-HSA-3301854 Reactome: R-HSA-9648025 |
| Cellular Localisation | Cytoplasm Nucleus Nucleus Speckle Cytoskeleton Microtubule Organizing Center Centrosome Spindle Pole Colocalizes With Apbb1 At The Nuclear Speckles Colocalizes With Pin1 In The Nucleus Colocalizes With Atf4 Cir1 Arhgap33 Ankra2 Cdc42 Nek9 Rad26l Rbbp6 Rps7 Trip4 Relb And Phf1 In The Centrosome Localizes To Spindle Microtubules In Metaphase And Anaphase And To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek6 antibody Anti-Never In Mitosis A-Related Kinase 6 antibody Anti-Nima-Related Protein Kinase 6 antibody Anti-Protein Kinase Sid6-1512 antibody Anti-NEK6 antibody |
Information sourced from Uniprot.org