Anti-NEIL3 antibody (491-540) (STJ94396)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human NEIL3. AA range:491-540
General Information
| Short Description | Rabbit polyclonal anti-Endonuclease 8-like 3 (491-540) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEIL3 |
| Gene ID | 55247 |
| Uniprot ID | NEIL3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NEIL3. AA range:491-540 |
| Immunogen Region | 491-540 |
| Specificity | NEIL3 Polyclonal Antibody detects endogenous levels of NEIL3 protein. |
Additional Info
| Tissue Specificity | Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts. |
| Function | DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA.also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. |
| Protein Name | Endonuclease 8-Like 3Dna Glycosylase Fpg2Dna Glycosylase/Ap Lyase Neil3Endonuclease Viii-Like 3Nei-Like Protein 3 |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110330Reactome: R-HSA-110331Reactome: R-HSA-9629232Reactome: R-HSA-9636003 |
| Cellular Localisation | NucleusChromosomeRecruited To Replication Stress Sites Via Interaction With Ubiquitinated Cmg Helicase |
| Alternative Antibody Names | Anti-Endonuclease 8-Like 3 antibodyAnti-Dna Glycosylase Fpg2 antibodyAnti-Dna Glycosylase/Ap Lyase Neil3 antibodyAnti-Endonuclease Viii-Like 3 antibodyAnti-Nei-Like Protein 3 antibodyAnti-NEIL3 antibody |
Information sourced from Uniprot.org