• Western blot analysis of lysates from HeLa, HepG2, HUVEC, and COLO cells, using NEIL3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using NEIL3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-NEIL3 antibody (491-540) (STJ94396)

SKU:
STJ94396

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Endonuclease 8-like 3 (491-540) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEIL3
Gene ID: 55247
Uniprot ID: NEIL3_HUMAN
Immunogen Region: 491-540
Specificity: NEIL3 Polyclonal Antibody detects endogenous levels of NEIL3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human NEIL3. AA range:491-540
Tissue Specificity Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
Function DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA.also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues.
Protein Name Endonuclease 8-Like 3
Dna Glycosylase Fpg2
Dna Glycosylase/Ap Lyase Neil3
Endonuclease Viii-Like 3
Nei-Like Protein 3
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-9629232
Reactome: R-HSA-9636003
Cellular Localisation Nucleus
Chromosome
Recruited To Replication Stress Sites Via Interaction With Ubiquitinated Cmg Helicase
Alternative Antibody Names Anti-Endonuclease 8-Like 3 antibody
Anti-Dna Glycosylase Fpg2 antibody
Anti-Dna Glycosylase/Ap Lyase Neil3 antibody
Anti-Endonuclease Viii-Like 3 antibody
Anti-Nei-Like Protein 3 antibody
Anti-NEIL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance