• Human cerebellum stained with anti-Neurofilament antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of neurons and neuronal processes.

Anti-NEFH antibody [2F11] (STJ180193)

SKU:
STJ180193

£129.50 - £385.50
Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-NEFH is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 2F11
Conjugation: Unconjugated
Isotype: IgG1/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:50‐200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: NEFH
Gene ID: 4744
Uniprot ID: NFH_HUMAN
Specificity: Positive control: Brain
Immunogen: Human NF-H isolated brain cells
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Heavy Polypeptide
Nf-H
200 Kda Neurofilament Protein
Neurofilament Triplet H Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Heavy Polypeptide antibody
Anti-Nf-H antibody
Anti-200 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet H Protein antibody
Anti-NEFH antibody
Anti-KIAA0845 antibody
Anti-NFH antibody

Information sourced from Uniprot.org

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