| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-NEDD8 (N-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC (both on frozen and paraffin embedded tissues) , WB. Use at a concentration of 10-50 µg/ml. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Nedd8 |
| Gene ID: |
18002 |
| Uniprot ID: |
NEDD8_MOUSE |
| Immunogen Region: |
N-Term |
| Specificity: |
Specific for NEDD8. |
| Immunogen: |
A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human, rat, zebrafish, drosophila and xenopus. |
| Post Translational Modifications | Cleavage of precursor form by UCHL3 or SENP8 is necessary for function. |
| Function | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. |
| Protein Name | Ubiquitin-Like Protein Nedd8NeddylinNeural Precursor Cell Expressed Developmentally Down-Regulated Protein 8Nedd-8 |
| Database Links | Reactome: R-MMU-2173789Reactome: -MMU-5689603Reactome: -MMU-8856825Reactome: -MMU-8951664Reactome: -MMU-917937 |
| Cellular Localisation | NucleusMainly Nuclear |
| Alternative Antibody Names | Anti-Ubiquitin-Like Protein Nedd8 antibodyAnti-Neddylin antibodyAnti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8 antibodyAnti-Nedd-8 antibodyAnti-Nedd8 antibodyAnti-Nedd-8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
