| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NEDD4L (836-955) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NEDD4L |
| Gene ID: |
23327 |
| Uniprot ID: |
NED4L_HUMAN |
| Immunogen Region: |
836-955 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 836-955 of human NEDD4L (NP_056092.2). |
| Immunogen Sequence: |
GDVDVNDWRQHSIYKNGYCP NHPVIQWFWKAVLLMDAEKR IRLLQFVTGTSRVPMNGFAE LYGSNGPQLFTIEQWGSPEK LPRAHTCFNRLDLPPYETFE DLREKLLMAVENAQGFEGVD |
| Tissue Specificity | Ubiquitously expressed, with highest levels in prostate, pancreas, and kidney. Expressed in melanocytes. |
| Post Translational Modifications | Phosphorylated by SGK1 or PKA.which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation. Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels. |
| Function | E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF. Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3. |
| Protein Name | E3 Ubiquitin-Protein Ligase Nedd4-LikeHect-Type E3 Ubiquitin Transferase Ned4lNedd4.2Nedd4-2 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-2173788Reactome: R-HSA-2173795Reactome: R-HSA-2672351Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmGolgi ApparatusEndosomeMultivesicular BodyMay Be Recruited To Exosomes By Ndfip1 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Nedd4-Like antibodyAnti-Hect-Type E3 Ubiquitin Transferase Ned4l antibodyAnti-Nedd4.2 antibodyAnti-Nedd4-2 antibodyAnti-NEDD4L antibodyAnti-KIAA0439 antibodyAnti-NEDL3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
