• Immunohistochemistry of mouse Colon with Anti-NEDD4 primary antibody (STJ501882) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-NEDD4 antibody (840-880) (STJ501882)

SKU:
STJ501882-100

£441.50
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Host: Rabbit
Applications: ELISA/IHC/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEDD4 (840-880) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.6-0.75 µg/µl
Dilution Range: WB: 1:250
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:200
IHC: 1:50-1:150
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Nedd4
Gene ID: 25489
Uniprot ID: NEDD4_RAT
Immunogen Region: 840-880
Immunogen: Synthetic peptide taken within amino acid region 840-880 on rat Nedd4 protein.
Tissue Specificity Ubiquitously expressed. Expression is highest in lung, kidney and brain.
Post Translational Modifications Undergoes 'Lys-29'-linked auto-ubiquitination at Lys-847 and serves as a scaffold for recruiting USP13 to form an NEDD4-USP13 deubiquitination complex.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Ubiquitinates DAZAP2, leading to its proteasomal degradation. Ubiquitinates POLR2A. Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the 'Lys-48'-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes.
Protein Name E3 Ubiquitin-Protein Ligase Nedd4
Hect-Type E3 Ubiquitin Transferase Nedd4
Database Links Reactome: R-RNO-1169408
Reactome: -RNO-1253288
Reactome: -RNO-8948747
Reactome: -RNO-8948751
Reactome: -RNO-983168
Cellular Localisation Cytoplasm
Nucleus
Cell Membrane
Peripheral Membrane Protein
Predominantly Cytoplasmic But Also Located In The Nucleus
Recruited To The Plasma Membrane By Grb10
Once Complexed With Grb10 And Igf1r
Follows Igf1r Internalization
Remaining Associated With Early Endosomes
Uncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal Compartment
May Be Recruited To Exosomes By Ndfip1
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Nedd4 antibody
Anti-Hect-Type E3 Ubiquitin Transferase Nedd4 antibody
Anti-Nedd4 antibody
Anti-Nedd4a antibody
Anti-Rpf1 antibody

Information sourced from Uniprot.org

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