• Western blot analysis of extracts of mouse skeletal muscle, using NDUFS7 antibody (STJ110172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFS7 antibody (39-213) (STJ110172)

SKU:
STJ110172

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS7 (39-213) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS7
Gene ID: 374291
Uniprot ID: NDUS7_HUMAN
Immunogen Region: 39-213
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 39-213 of human NDUFS7 (NP_077718.3).
Immunogen Sequence: PSSTQPALPKARAVAPKPSS RGEYVVAKLDDLVNWARRSS LWPMTFGLACCAVEMMHMAA PRYDMDRFGVVFRASPRQSD VMIVAGTLTNKMAPALRKVY DQMPEPRYVVSMGSCANGGG YYHYSYSVVRGCDRIVPVDI YIPGCPPTAEALLYGILQLQ RKIKRERRLQIWYRR
Post Translational Modifications Hydroxylated ar Arg-111 by NDUFAF5 early in the pathway of assembly of complex I, before the formation of the juncture between peripheral and membrane arms.
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 7 - Mitochondrial
Complex I-20kd
Ci-20kd
Nadh-Ubiquinone Oxidoreductase 20 Kda Subunit
Psst Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 7 - Mitochondrial antibody
Anti-Complex I-20kd antibody
Anti-Ci-20kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 20 Kda Subunit antibody
Anti-Psst Subunit antibody
Anti-NDUFS7 antibody

Information sourced from Uniprot.org

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