• Immunofluorescence analysis of U2OS cells using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NDUFB8 in paraffin-embedded mouse kidney using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFB8 in paraffin-embedded human liver using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFB8 antibody (29-186) (STJ11103314)

SKU:
STJ11103314

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB8 (29-186) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB8
Gene ID: 4714
Uniprot ID: NDUB8_HUMAN
Immunogen Region: 29-186
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-186 of human NDUFB8 (NP_004995.1).
Immunogen Sequence: ASHMTKDMFPGPYPRTPEER AAAAKKYNMRVEDYEPYPDD GMGYGDYPKLPDRSQHERDP WYSWDQPGLRLNWGEPMHWH LDMYNRNRVDTSPTPVSWHV MCMQLFGFLAFMIFMCWVGD VYPVYQPVGPKQYPYNNLYL ERGGDPSKEPERVVHYEI
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 8 - Mitochondrial
Complex I-Ashi
Ci-Ashi
Nadh-Ubiquinone Oxidoreductase Ashi Subunit
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 8 - Mitochondrial antibody
Anti-Complex I-Ashi antibody
Anti-Ci-Ashi antibody
Anti-Nadh-Ubiquinone Oxidoreductase Ashi Subunit antibody
Anti-NDUFB8 antibody

Information sourced from Uniprot.org

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