• Immunofluorescence analysis of L929 cells using NDUFAF2 Rabbit polyclonal antibody (STJ116508) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFAF2 Rabbit polyclonal antibody (STJ116508) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFAF2 antibody (1-169) (STJ116508)

SKU:
STJ116508

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFAF2
Gene ID: 91942
Uniprot ID: NDUF2_HUMAN
Immunogen Region: 1-169
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human NDUFAF2 (NP_777549.1).
Immunogen Sequence: MGWSQDLFRALWRSLSREVK EHVGTDQFGNKYYYIPQYKN WRGQTIREKRIVEAANKKEV DYEAGDIPTEWEAWIRRTRK TPPTMEEILKNEKHREEIKI KSQDFYEKEKLLSKETSEEL LPPPVQTQIKGHASAPYFGK EEPSVAPSSTGKTFQPGSWM PRDGKSHNQ
Tissue Specificity Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts.
Function Acts as a molecular chaperone for mitochondrial complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 2
B17.2-Like
B17.2l
Mimitin
Myc-Induced Mitochondrial Protein
Mmtn
Ndufa12-Like Protein
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 2 antibody
Anti-B17.2-Like antibody
Anti-B17.2l antibody
Anti-Mimitin antibody
Anti-Myc-Induced Mitochondrial Protein antibody
Anti-Mmtn antibody
Anti-Ndufa12-Like Protein antibody
Anti-NDUFAF2 antibody
Anti-NDUFA12L antibody

Information sourced from Uniprot.org

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