Anti-NCL antibody (300-466) (STJ29927)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NCL (300-466) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NCL |
| Gene ID | 4691 |
| Uniprot ID | NUCL_HUMAN |
| Immunogen Region | 300-466 |
| Immunogen Sequence | GTEPTTAFNLFVGNLNFNKS APELKTGISDVFAKNDLAVV DVRIGMTRKFGYVDFESAED LEKALELTGLKVFGNEIKLE KPKGKDSKKERDARTLLAKN LPYKVTQDELKEVFEDAAEI RLVSKDGKSKGIAYIEFKTE ADAEKTFEEKQGTEIDGRSI SLYYTGE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 300-466 of human NCL (NP_005372.2). |
Additional Info
| Post Translational Modifications | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Symmetrically methylated by PRMT5. |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Protein Name | NucleolinProtein C23 |
| Database Links | Reactome: R-HSA-6791226Reactome: R-HSA-9820960 |
| Cellular Localisation | NucleusNucleolusCytoplasmLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Nucleolin antibodyAnti-Protein C23 antibodyAnti-NCL antibody |
Information sourced from Uniprot.org