| Host: | Goat |
| Applications: | Pep-ELISA/IF/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-NCKAP1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5µg/ml antibody staining, and was successfully blocked by incu |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NCKAP1 |
| Gene ID: | 10787 |
| Uniprot ID: | NCKP1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_038464.1; NP_995314.1 |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1). |
| Immunogen Sequence: | TPREYLTSHLEIR |
| Function | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. |
| Protein Name | Nck-Associated Protein 1Nap 1Membrane-Associated Protein Hem-2P125nap1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinCytoplasmic SideCell ProjectionLamellipodium MembraneAt The Interface Between The Lamellipodial Actin Meshwork And The Membrane |
| Alternative Antibody Names | Anti-Nck-Associated Protein 1 antibodyAnti-Nap 1 antibodyAnti-Membrane-Associated Protein Hem-2 antibodyAnti-P125nap1 antibodyAnti-NCKAP1 antibodyAnti-HEM2 antibodyAnti-KIAA0587 antibodyAnti-NAP1 antibody |
Information sourced from Uniprot.org
