Anti-NCE2 antibody [R05-8C4] (STJA0034607)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human NCE2
General Information
| Short Description | Rabbit monoclonal anti-NCE2 for use in WB, IHC-F, IHC-P, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-8C4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | UBE2F |
| Gene ID | 140739 |
| Uniprot ID | UBE2F_HUMAN |
| Immunogen | Recombinant protein of human NCE2 |
Additional Info
| Tissue Specificity | Widely expressed (at protein level). |
| Post Translational Modifications | The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5). |
| Protein Name | Nedd8-Conjugating Enzyme Ube2fNedd8 Carrier Protein Ube2fNedd8 Protein Ligase Ube2fNedd8-Conjugating Enzyme 2Ring-Type E3 Nedd8 Transferase Ube2fUbiquitin-Conjugating Enzyme E2 F |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nedd8-Conjugating Enzyme Ube2f antibodyAnti-Nedd8 Carrier Protein Ube2f antibodyAnti-Nedd8 Protein Ligase Ube2f antibodyAnti-Nedd8-Conjugating Enzyme 2 antibodyAnti-Ring-Type E3 Nedd8 Transferase Ube2f antibodyAnti-Ubiquitin-Conjugating Enzyme E2 F antibodyAnti-UBE2F antibodyAnti-NCE2 antibody |
Information sourced from Uniprot.org