| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NCBP2 (1-156) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NCBP2 |
| Gene ID: |
22916 |
| Uniprot ID: |
NCBP2_HUMAN |
| Immunogen Region: |
1-156 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2). |
| Immunogen Sequence: |
MSGGLLKALRSDSYVELSQY RDQHFRGDNEEQEKLLKKSC TLYVGNLSFYTTEEQIYELF SKSGDIKKIIMGLDKMKKTA CGFCFVEYYSRADAENAMRY INGTRLDDRIIRTDWDAGFK EGRQYGRGRSGGQVRDEYRQ DYDAGRGGYGKLAQNQ |
| Function | Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus. |
| Protein Name | Nuclear Cap-Binding Protein Subunit 220 Kda Nuclear Cap-Binding ProteinCell Proliferation-Inducing Gene 55 ProteinNcbp 20 Kda SubunitCbp20Ncbp-Interacting Protein 1Nip1 |
| Database Links | Reactome: R-HSA-111367Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167242Reactome: R-HSA-191859Reactome: R-HSA-674695Reactome: R-HSA-6803529Reactome: R-HSA-6807505Reactome: R-HSA-72086Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856Reactome: R-HSA-77588Reactome: R-HSA-77595Reactome: R-HSA-8851708Reactome: R-HSA-9010553Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Nuclear Cap-Binding Protein Subunit 2 antibodyAnti-20 Kda Nuclear Cap-Binding Protein antibodyAnti-Cell Proliferation-Inducing Gene 55 Protein antibodyAnti-Ncbp 20 Kda Subunit antibodyAnti-Cbp20 antibodyAnti-Ncbp-Interacting Protein 1 antibodyAnti-Nip1 antibodyAnti-NCBP2 antibodyAnti-CBP20 antibodyAnti-PIG55 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ23076) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/99355/384261/STJ23076_1__86905.1713152939.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ23076) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
