• Western blot analysis of extracts of various cell lines, using Alpha SNAP antibody (STJ110255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using Alpha SNAP antibody (STJ110255) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Alpha SNAP antibody (STJ110255) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Alpha SNAP antibody (STJ110255) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Alpha SNAP antibody (STJ110255) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Alpha SNAP antibody (STJ110255) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate using Alpha SNAP antibody (STJ110255) (40x lens).
  • Western blot analysis of extracts of various cell lines, using Alpha SNAP antibody (STJ110255) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NAPA antibody (1-295) (STJ110255)

SKU:
STJ110255

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAPA (1-295) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAPA
Gene ID: 8775
Uniprot ID: SNAA_HUMAN
Immunogen Region: 1-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human Alpha SNAP (NP_003818.2).
Immunogen Sequence: MDNSGKEAEAMALLAEAERK VKNSQSFFSGLFGGSSKIEE ACEIYARAANMFKMAKNWSA AGNAFCQAAQLHLQLQSKHD AATCFVDAGNAFKKADPQEA INCLMRAIEIYTDMGRFTIA AKHHISIAEIYETELVDIEK AIAHYEQSADYYKGEESNSS ANKCLLKVAGYAALLEQYQK AIDIYEQVGTNAMDSPLLKY SAKDYFFKAALCHFCIDMLN AKLAVQKYEELFPAFSDSR
Function Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane.
Protein Name Alpha-Soluble Nsf Attachment Protein
Snap-Alpha
N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha
Database Links Reactome: R-HSA-204005
Reactome: R-HSA-432722
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811438
Reactome: R-HSA-6811440
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Alpha-Soluble Nsf Attachment Protein antibody
Anti-Snap-Alpha antibody
Anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein Alpha antibody
Anti-NAPA antibody
Anti-SNAPA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance