• Western blot analysis of extracts of various cell lines, using NAGA antibody (STJ111983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of L929 cells using NAGA Rabbit polyclonal antibody (STJ111983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NAGA rabbit polyclonal antibody (STJ111983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NAGA antibody (STJ111983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NAGA antibody (312-411) (STJ111983)

SKU:
STJ111983

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NAGA (312-411) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NAGA
Gene ID: 4668
Uniprot ID: NAGAB_HUMAN
Immunogen Region: 312-411
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 312-411 of human NAGA (NP_000253.1).
Immunogen Sequence: IQGRRIHKEKSLIEVYMRPL SNKASALVFFSCRTDMPYRY HSSLGQLNFTGSVIYEAQDV YSGDIISGLRDETNFTVIIN PSGVVMWYLYPIKNLEMSQQ
Function Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.
Protein Name Alpha-N-Acetylgalactosaminidase
Alpha-Galactosidase B
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Alpha-N-Acetylgalactosaminidase antibody
Anti-Alpha-Galactosidase B antibody
Anti-NAGA antibody

Information sourced from Uniprot.org

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