Anti-NADPH oxidase 4 (NOX4) antibody [SRM13305] (STJ11108035)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Nadph Oxidase 4 antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM13305 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB, 1:10000-1:20000IHC-P, 1:200-1:2000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOX4 |
| Gene ID | 50507 |
| Uniprot ID | NOX4_HUMAN |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Immunogen Sequence | Human |
Additional Info
| Post Translational Modifications | Deubiquitinated by USP19. Isoform 4: N-glycosylated and glycosylation is required for its proper function. Isoform 3: N-glycosylated. |
| Function | NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Can also catalyze to a smaller extent, the reduction of oxygen to superoxide. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release. Isoform 4: NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in redox signaling in vascular cells. Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Isoform 3: Lacks superoxide-generating NADPH oxidase activity. |
| Protein Name | Nadph Oxidase 4Kidney Oxidase-1Kox-1Kidney Superoxide-Producing Nadph OxidaseRenal Nad(Ph-Oxidase |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-9702518Reactome: R-HSA-9703465 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembraneMulti-Pass Membrane ProteinCell MembraneCell JunctionFocal AdhesionNucleusIsoform 4: NucleusNucleolusPerinuclear RegionIsoform 3: CytoplasmIsoform 5: CytoplasmIsoform 6: Cytoplasm |
| Alternative Antibody Names | Anti-Nadph Oxidase 4 antibodyAnti-Kidney Oxidase-1 antibodyAnti-Kox-1 antibodyAnti-Kidney Superoxide-Producing Nadph Oxidase antibodyAnti-Renal Nad(Ph-Oxidase antibodyAnti-NOX4 antibodyAnti-RENOX antibody |
Information sourced from Uniprot.org