| Host: | Mouse |
| Applications: | WB/ICC/IHC/IP/FC |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-His Tag (C-Term) is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Flow Cytometry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | M050 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:5000IHC 1:100ICC 1:100IP 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: | C-Term |
| Specificity: | Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunopreci |
| Immunogen: | Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag. |
| Background | Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino-or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays. |
Information sourced from Uniprot.org
