| Short Description : | Rabbit polyclonal anti-Myosin Light Chain (N-Term) for use in ELISA, WB and ICC in Chicken, Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/ICC |
| Host: | Rabbit |
| Reactivity: | Chicken/Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000ICC 1:200 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction: | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
| Gene Symbol: | MYL12A |
| Gene ID: | 10627 |
| Uniprot ID: | ML12A_HUMAN |
| Immunogen: | MLC synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminus of human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in oth |
| Immunogen Region: | N-Term |
| Immunogen Sequence: | Human |
| Specificity: | The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in mouse C2C12, rat PC12, and human A431. |
| Post Translational Modifications | Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. |
| Function | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. |
| Protein Name | Myosin Regulatory Light Chain 12aEpididymis Secretory Protein Li 24Hel-S-24Mlc-2bMyosin RlcMyosin Regulatory Light Chain 2 - NonsarcomericMyosin Regulatory Light Chain Mrlc3 |
| Database Links | Reactome: R-HSA-3928664Reactome: R-HSA-445355 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Myosin Regulatory Light Chain 12a antibodyAnti-Epididymis Secretory Protein Li 24 antibodyAnti-Hel-S-24 antibodyAnti-Mlc-2b antibodyAnti-Myosin Rlc antibodyAnti-Myosin Regulatory Light Chain 2 - Nonsarcomeric antibodyAnti-Myosin Regulatory Light Chain Mrlc3 antibodyAnti-MYL12A antibodyAnti-MLCB antibodyAnti-MRLC3 antibodyAnti-RLC antibody |
Information sourced from Uniprot.org
