• Western blot analysis of 1, mouse-heart 2, Hela cells using primary antibody diluted at 1:500 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-MYL2 antibody (91-140 aa) (STJ99335)

SKU:
STJ99335

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (91-140 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYL2
Gene ID: 4633
Uniprot ID: MLRV_HUMAN
Immunogen Region: 91-140 aa
Specificity: MYL2 Polyclonal Antibody detects endogenous levels of MYL2
Immunogen: Synthesized peptide derived from the human MYL2 at the amino acid range 91-140
Post Translational Modifications N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3 and MYLK2.promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B. The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation).regulates cardiac torsion and workload distribution.
Function Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion.as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly.
Protein Name Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle Isoform
Mlc-2
Mlc-2v
Cardiac Myosin Light Chain 2
Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle Isoform
Mlc-2s/V
Ventricular Myosin Light Chain 2
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
A Band
Alternative Antibody Names Anti-Myosin Regulatory Light Chain 2 - Ventricular/Cardiac Muscle Isoform antibody
Anti-Mlc-2 antibody
Anti-Mlc-2v antibody
Anti-Cardiac Myosin Light Chain 2 antibody
Anti-Myosin Light Chain 2 - Slow Skeletal/Ventricular Muscle Isoform antibody
Anti-Mlc-2s/V antibody
Anti-Ventricular Myosin Light Chain 2 antibody
Anti-MYL2 antibody
Anti-MLC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance