Western blot analysis of extracts of various cell lines, using MYL12A antibody (STJ113619) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-MYL12A antibody (92-171) (STJ113619)

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STJ113619
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYL12A
Gene ID: 10627
Uniprot ID: ML12A_HUMAN
Immunogen Region: 92-171
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 92-171 of human MYL12A (NP_006462.1).
Immunogen Sequence: KLNGTDPEDVIRNAFACFDE EATGTIQEDYLRELLTTMGD RFTDEEVDELYREAPIDKKG NFNYIEFTRILKHGAKDKDD
Post Translational Modifications Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Function Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Protein Name Myosin Regulatory Light Chain 12a
Epididymis Secretory Protein Li 24
Hel-S-24
Mlc-2b
Myosin Rlc
Myosin Regulatory Light Chain 2 - Nonsarcomeric
Myosin Regulatory Light Chain Mrlc3
Database Links Reactome: R-HSA-3928664
Reactome: R-HSA-445355
Alternative Antibody Names Anti-Myosin Regulatory Light Chain 12a antibody
Anti-Epididymis Secretory Protein Li 24 antibody
Anti-Hel-S-24 antibody
Anti-Mlc-2b antibody
Anti-Myosin Rlc antibody
Anti-Myosin Regulatory Light Chain 2 - Nonsarcomeric antibody
Anti-Myosin Regulatory Light Chain Mrlc3 antibody
Anti-MYL12A antibody
Anti-MLCB antibody
Anti-MRLC3 antibody
Anti-RLC antibody

Information sourced from Uniprot.org

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