| Host: |
Rabbit |
| Applications: |
WB/ICC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Myosin IIA Heavy Chain (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:2000IHCICC 1:100IP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MYH9 |
| Gene ID: |
4627 |
| Uniprot ID: |
MYH9_HUMAN |
| Immunogen Region: |
C-Term |
| Specificity: |
The antibody detects a 200 kDa* protein corresponding to the apparent molecular mass of NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. |
| Immunogen: |
NMHC-IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not we |
| Tissue Specificity | In the kidney, expressed in the glomeruli. Also expressed in leukocytes. |
| Post Translational Modifications | ISGylated. Ubiquitination. |
| Function | Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction.this function is mechanically antagonized by MYH10. |
| Protein Name | Myosin-9Cellular Myosin Heavy Chain - Type AMyosin Heavy Chain 9Myosin Heavy Chain - Non-Muscle IiaNon-Muscle Myosin Heavy Chain ANmmhc-ANon-Muscle Myosin Heavy Chain IiaNmmhc Ii-ANmmhc-Iia |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-2029482Reactome: R-HSA-3928663Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123Reactome: R-HSA-9662360Reactome: R-HSA-9662361Reactome: R-HSA-9662834Reactome: R-HSA-9664422Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmCytoskeletonCell CortexCytoplasmic VesicleSecretory VesicleCortical GranuleColocalizes With Actin Filaments At Lamellipodia Margins And At The Leading Edge Of Migrating CellsIn Retinal Pigment Epithelial CellsPredominantly Localized To Stress Fiber-Like Structures With Some Localization To Cytoplasmic Puncta |
| Alternative Antibody Names | Anti-Myosin-9 antibodyAnti-Cellular Myosin Heavy Chain - Type A antibodyAnti-Myosin Heavy Chain 9 antibodyAnti-Myosin Heavy Chain - Non-Muscle Iia antibodyAnti-Non-Muscle Myosin Heavy Chain A antibodyAnti-Nmmhc-A antibodyAnti-Non-Muscle Myosin Heavy Chain Iia antibodyAnti-Nmmhc Ii-A antibodyAnti-Nmmhc-Iia antibodyAnti-MYH9 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
