• Immunofluorescence analysis of RD cells using MYH7/Beta-MHC Rabbit polyclonal antibody (STJ29702) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using MYH7/Beta-MHC Rabbit polyclonal antibody (STJ29702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MYH7 antibody (1728-1744) (STJ29702)

SKU:
STJ29702

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH7
Gene ID: 4625
Uniprot ID: MYH7_HUMAN
Immunogen Region: 1728-1744
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1700-1800 of human MYH7/Beta-MHC (NP_000248.2).
Immunogen Sequence: KLAEQELIETSERVQLLHSQ NTSLINQKKKMDADLSQLQT EVEEAVQECRNAEEKAKKAI TDAAMMAEELKKEQDTSAHL ERMKKNMEQTIKDLQHRLDE AEQIALKGGKKQLQKLEARV RELENELEAEQKRNAESVKG MRKSERRIKELTYQTEEDRK NLLRLQDLVDKLQLKVKAYK RQAEEAEEQANTNLSKFRKV QHELDEAEERADIAESQVNK LRAKSRDIGTKGLNEE
Tissue Specificity Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
Function Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
Protein Name Myosin-7
Myosin Heavy Chain 7
Myosin Heavy Chain Slow Isoform
Myhc-Slow
Myosin Heavy Chain - Cardiac Muscle Beta Isoform
Myhc-Beta
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-7 antibody
Anti-Myosin Heavy Chain 7 antibody
Anti-Myosin Heavy Chain Slow Isoform antibody
Anti-Myhc-Slow antibody
Anti-Myosin Heavy Chain - Cardiac Muscle Beta Isoform antibody
Anti-Myhc-Beta antibody
Anti-MYH7 antibody
Anti-MYHCB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance