• Western blot analysis of extracts of Rat skeletal muscle, using MYH2 antibody (STJ117487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of mouse skeletal muscle, using MYH2 antibody (STJ117487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of mouse skeletal muscle cells using MYH2 rabbit polyclonal antibody (STJ117487) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat skeletal muscle cells using MYH2 rabbit polyclonal antibody (STJ117487) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse skeletal muscle cells using MYH2 rabbit polyclonal antibody (STJ117487) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat skeletal muscle cells using MYH2 rabbit polyclonal antibody (STJ117487) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MYH2 antibody (621-635) (STJ117487)

SKU:
STJ117487

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH2
Gene ID: 4620
Uniprot ID: MYH2_HUMAN
Immunogen Region: 621-635
Specificity: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human MYH2 (NP_001093582.1).
Immunogen Sequence: NKDPLNETVVGLYQKSAMKT LAQLFSGAQTAEGEGAGGGA KKGGKKKGSSFQTVSALFRE NLNKLMTNLRSTHPHFVRCI IPNETKTPGAMEHELVLHQL RCNGVLEGIRICRKGFPSRI LYADFKQRYKVLNASAIPEG QFIDSKKASEKLLASIDIDH TQYKFGHTKVFFKAGLLGLL EEMRDDKLAQLITRTQARCR GFLARVEYQRMVERREAIFC IQYNIRSFMNVKHWPWMKL
Function Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction.
Protein Name Myosin-2
Myosin Heavy Chain 2
Myosin Heavy Chain 2a
Myhc-2a
Myosin Heavy Chain Iia
Myhc-Iia
Myosin Heavy Chain - Skeletal Muscle - Adult 2
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Myofibril
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-2 antibody
Anti-Myosin Heavy Chain 2 antibody
Anti-Myosin Heavy Chain 2a antibody
Anti-Myhc-2a antibody
Anti-Myosin Heavy Chain Iia antibody
Anti-Myhc-Iia antibody
Anti-Myosin Heavy Chain - Skeletal Muscle - Adult 2 antibody
Anti-MYH2 antibody
Anti-MYHSA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance