• Immunofluorescence analysis of paraffin-embedded rat heart using MYH1 Rabbit polyclonal antibody (STJ29015) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse heart using MYH1 Rabbit polyclonal antibody (STJ29015) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Human heart using MYH1 Rabbit polyclonal antibody (STJ29015) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MYH1 in paraffin-embedded Human skeletal muscle using MYH1 Rabbit polyclonal antibody (STJ29015) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit polyclonal antibody (STJ29015) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:3s.
  • Western blot analysis of various lysates using MYH1 Rabbit polyclonal antibody (STJ29015) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MYH1 antibody (1-100) (STJ29015)

SKU:
STJ29015

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYH1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH1
Gene ID: 4619
Uniprot ID: MYH1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3).
Immunogen Sequence: MSSDSEMAIFGEAAPFLRKS ERERIEAQNKPFDAKTSVFV VDPKESFVKATVQSREGGKV TAKTEAGATVTVKDDQVFPM NPPKYDKIEDMAMMTHLHEP
Function Muscle contraction.
Protein Name Myosin-1
Myosin Heavy Chain 1
Myosin Heavy Chain 2x
Myhc-2x
Myosin Heavy Chain Iix/D
Myhc-Iix/D
Myosin Heavy Chain - Skeletal Muscle - Adult 1
Cellular Localisation Cytoplasm
Myofibril
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-1 antibody
Anti-Myosin Heavy Chain 1 antibody
Anti-Myosin Heavy Chain 2x antibody
Anti-Myhc-2x antibody
Anti-Myosin Heavy Chain Iix/D antibody
Anti-Myhc-Iix/D antibody
Anti-Myosin Heavy Chain - Skeletal Muscle - Adult 1 antibody
Anti-MYH1 antibody

Information sourced from Uniprot.org

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