• Western blot analysis of Mouse skeletal muscle, using MUSK antibody (STJ24645) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MUSK antibody (424-783) (STJ24645)

SKU:
STJ24645

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MUSK (424-783) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MUSK
Gene ID: 4593
Uniprot ID: MUSK_HUMAN
Immunogen Region: 424-783
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 424-783 of human MUSK (NP_001159752.1).
Immunogen Sequence: LTITTLYCCRRRKQWKNKKR ESAAVTLTTLPSELLLDRLH PNPMYQRMPLLLNPKLLSLE YPRNNIEYVRDIGEGAFGRV FQARAPGLLPYEPFTMVAVK MLKEEASADMQADFQREAAL MAEFDNPNIVKLLGVCAVGK PMCLLFEYMAYGDLNEFLRS MSPHTVCSLSHSDLSMRAQV SSPGPPPLSCAEQLCIARQV AAGMAYLSERKFVHRDLATR NCLVGENMVVKIADFGLSR
Post Translational Modifications Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation. Phosphorylated. Phosphorylation is induced by AGRIN in a LRP4-dependent manner. Autophosphorylated. Autophosphorylation at Tyr-554 is required for interaction with DOK7 which in turn stimulates the phosphorylation and the activation of MUSK. Neddylated.
Function Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
Protein Name Muscle - Skeletal Receptor Tyrosine-Protein Kinase
Muscle-Specific Tyrosine-Protein Kinase Receptor
Musk
Muscle-Specific Kinase Receptor
Database Links Reactome: R-HSA-3000178
Cellular Localisation Postsynaptic Cell Membrane
Single-Pass Type I Membrane Protein
Colocalizes With Acetylcholine Receptors (Achr) To The Postsynaptic Cell Membrane Of The Neuromuscular Junction
Alternative Antibody Names Anti-Muscle - Skeletal Receptor Tyrosine-Protein Kinase antibody
Anti-Muscle-Specific Tyrosine-Protein Kinase Receptor antibody
Anti-Musk antibody
Anti-Muscle-Specific Kinase Receptor antibody
Anti-MUSK antibody

Information sourced from Uniprot.org

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