| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Munc13-4/UNC13D (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 h |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | UNC13D |
| Gene ID: | 201294 |
| Uniprot ID: | UN13D_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_954712.1 |
| Immunogen Sequence: | ETQKHKKDLHPLFD |
| Function | Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells. |
| Protein Name | Protein Unc-13 Homolog DMunc13-4 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinLate EndosomeRecycling EndosomeLysosomeColocalizes With Cytotoxic Granules At The Plasma MembraneLocalizes To Endosomal Exocytic Vesicles |
| Alternative Antibody Names | Anti-Protein Unc-13 Homolog D antibodyAnti-Munc13-4 antibodyAnti-UNC13D antibody |
Information sourced from Uniprot.org